Anti-Tiam1 Rabbit pAb

Analysis Note

Positive ControlJurkat or NIH3T3 cells

Application

Immunoblotting (1:1000)

General description

This Anti-Tiam1 Rabbit pAb is validated for use in Immunoblotting for the detection of Tiam1.

Recognizes the ~200 kDa Tiam1 protein in Jurkat and NIH3T3 cells.

Tiam1 (T-cell lymphoma invasion and metastasis 1) was originally identified by its ability to induce invasion by T-lymphoma cells when amplified. Tiam1 is a specific guanine nucleotide exchange factor (GEF) for the GTPase, Rac. By activating Rac1, Tiam1 regulates many cellular functions including actin cytoskeleton dynamics, cell proliferation, and cell survival. Tiam1 activity is regulated by its translocation to the membrane and the subsequent binding of its PH domain to phosphatidylinositol-3,4,5-triphosphate. Tiam1 is cleaved in a caspase-dependent manner in apoptotic cells, rendering it inactive as a result of an inability to translocate to the plasma membrane.

Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~200 kDa Tiam1 protein.

Immunogen

Human

a synthetic peptide corresponding to amino acids from the C-terminal domain of human Tiam1

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Mertens, A.E., et al. 2003. FEBS Lett.546, 11.Qi, H., et al. 2001. Cell Growth Differ.12, 603.Stam, J.C., et al. 1997. J. Biol. Chem.272, 28447.

Antibody should be titrated for optimal results in individual systems.

Packaging

50 µg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Physical form

In Tris-Citrate/phosphate, pH 7.0-8.0.

Reconstitution

Following initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.

Warning

Toxicity: Highly Toxic (H)